• Reference range: negative < 1:16; positive > 1:16
• Specimen: serum, CSF
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777
• Fee: $170
• Method Reference: Sabin AB, Feldman HA. Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science108:660-663, 1948.

• Reference range: negative 0.0-1.6, equivocal 1.7-1.9, positive > 2.0 (serum); negative 0.0 - 0.3, positive > 0.4 (CSF)
• Specimen: serum, CSF
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86778
• Fee: $175
• Method Reference: Naot Y, Remington J. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii : use for diagnosis of acute acquired toxoplasmosis. J Infect Dis 142:757-766, 1980

• Reference range: reported as positive or negative
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86778
• Fee: $175
• Method Reference: Desmonts G, Naot Y, Remington JS. Immunoglobulin M immunosorbent agglutination assay for diagnosis of acute congenital and acquired toxoplasma infections. J Clin Microbiol 14:486-491, 1981

• Reference range:
  • Patient > 6 months of age: negative 0.0 - 1.4, equivocal 1.5 - 2.0,
    positive > 2.1
  • Infants < 6 months of age: negative 0.0 - 0.9, positive > 1.0
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777-59
• Fee: $170
• Method Reference: Stepick-Biek P, Thulliez P, Araujo F, Remington JS. IgA antibodies for diagnosis of acute congenital and acquired toxoplasmosis. J Infect Dis 162:270-273, 1990

• Reference range: reported as negative, equivocal or positive
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777-59
• Fee: $165
• Method Reference: Wong S, Hadju M, Ramirez R, Thulliez P, McLeod R, Remington JS. Role of specific immunoglobulin E in diagnosis of acute toxoplasma infection and toxoplasmosis. J Clin Microbiol 31:2952-2959, 1993

• Reference range: refer to table
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86406 (2 units)
• Fee: $186
• Method Reference: Danneman B, Vaughn W, Thulliez P, Remington JS. Differential agglutination test for diagnosis of recently acquired infection with Toxoplasma gondii. J Clin Microbiol 28:1928-1933, 1990

• Reference range: high avidity excludes infection within the previous 4 months
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777-59
• Fee: $186 (Includes fee for IgG (Dye test) and IgM ELISA)
• Method Reference: Pelloux, H. et al. Determination of anti-Toxoplasma gondii immunoglobulin G avidity: adaption to the Vidas system (bioMérieux). Diagn Microbiol Infect Dis 32: 69-73, 1998

• Reference range: reported as "toxoplasma DNA detected" or "toxoplasma DNA not detected"
• Specimen: (refer to PCR Specimen Requirements table)
• Volume: (refer to PCR Specimen Requirements table)
• Transport temperature: (refer to PCR Specimen Requirements table)
• CPT code: 87798
• Fee: $435-$455 (refer to Fee Schedule)
• Method Reference: Grover C, Thulliez P, Remington J, Boothroyd J. Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. J Clin Microbiol 28:2297-2301, 1990.
  
  Parmley S, Goebel F, Remington JS. Detection of Toxoplasma gondii in cerebro-spinal fluid from AIDS patients by polymerase chain reaction. J Clin Microbiol 30:3000-3002, 1992

• Reference range: reported as positive or negative
• Specimen: whole blood (EDTA or Citrate preferred), amniotic fluid, placenta and other tissues
  
  NOTE: Specimens must not be frozen or placed in formalin or other fixative. Inoculations are recommended within 48 hours of specimen collection date. Results available in six weeks.
• Volume: 3 ml (fluids); 1 g (solid tissues)
• Transport temperature: 2 – 8° C.
• CPT code: 87003
• Fee: $627
• Method Reference: Remington J.S., McLeod R., Thulliez P., Desmonts G. Toxoplasmosis.
  

  
  In Remington J.S., Wilson C.B., Baker C.J. and Klein, J.O. (eds.) Infectious Diseases of the Fetus and Newborn Infant, Sixth Edition. Philadelphia: Elsevier Saunders Company, 2006, pp 1009-1010.

IgG (Dye Test), IgM ELISA or IgM ISAGA

• Specimen: serum
• Volume: 0.5 to 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $330

IgG (Dye Test), IgM ELISA, Avidity

• Specimen: serum
• Volume: 0.5 to 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $516

IgG (Dye Test), IgM ELISA, AC/HS

• Specimen: serum
• Volume: 0.5 to 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $516

IgG (Dye Test), IgM ISAGA, IgA ELISA

• Specimen: serum
• Volume: 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $495

IgG (Dye Test), IgM ELISA, IgA ELISA, IgE ELISA, AC/HS

• Specimen: serum
• Volume: 2.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $811