• Reference range: negative < 1:16; positive > 1:16
• Specimen: serum, CSF
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777
• Fee: $180
• Method Reference: Sabin AB, Feldman HA. Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science108:660-663, 1948.

• Reference range: negative < 4, equivocal ≥ 4 to < 8, positive ≥ 8
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777
• Fee: $180
• Method Reference: De Champs C, Pelloux H, Cambon M, Fricker-Hidalgo H, Goullier-Fleuret A, Ambroise-Thomas P. Evaluation of the second generation IMx Toxo IgG antibody assay for detection of antibodies to Toxoplasma gondii in human sera. J Clin Lab Anal. 1997;11(4):214-9. doi: 10.1002/(sici)1098-2825(1997)11:4<214::aid-jcla7>3.0.co;2-8.PMID: 9219063

• Reference range: negative 0.0-1.6, equivocal 1.7-1.9, positive > 2.0 (serum); negative 0.0 - 0.3, positive > 0.4 (CSF)
• Specimen: serum, CSF
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86778
• Fee: $185
• Method Reference: Naot Y, Remington J. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii : use for diagnosis of acute acquired toxoplasmosis. J Infect Dis 142:757-766, 1980

• Reference range: negative < 0 .55, equivocal ≥ 0.55 to < 0.65, positive ≥ 0.65
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86778
• Fee: $185
• Method Reference: De Champs C, Pelloux H, Cambon M, Fricker-Hidalgo H, Goullier-Fleuret A, Ambroise-Thomas P. Evaluation of the second generation IMx Toxo IgG antibody assay for detection of antibodies to Toxoplasma gondii in human sera. J Clin Lab Anal. 1997;11(4):214-9. doi: 10.1002/(sici)1098-2825(1997)11:4<214::aid-jcla7>3.0.co;2-8.PMID: 9219063

• Reference range: reported as positive or negative
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86778
• Fee: $185
• Method Reference: Desmonts G, Naot Y, Remington JS. Immunoglobulin M immunosorbent agglutination assay for diagnosis of acute congenital and acquired toxoplasma infections. J Clin Microbiol 14:486-491, 1981

• Reference range:
  • Patient > 6 months of age: negative 0.0 - 1.4, equivocal 1.5 - 2.0,
    positive > 2.1
  • Infants < 6 months of age: negative 0.0 - 0.9, positive > 1.0
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777-59
• Fee: $180
• Method Reference: Stepick-Biek P, Thulliez P, Araujo F, Remington JS. IgA antibodies for diagnosis of acute congenital and acquired toxoplasmosis. J Infect Dis 162:270-273, 1990

• Reference range: reported as negative, equivocal or positive
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777-59
• Fee: $175
• Method Reference: Wong S, Hadju M, Ramirez R, Thulliez P, McLeod R, Remington JS. Role of specific immunoglobulin E in diagnosis of acute toxoplasma infection and toxoplasmosis. J Clin Microbiol 31:2952-2959, 1993

• Reference range: refer to table
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86406 (2 units)
• Fee: $196
• Method Reference: Danneman B, Vaughn W, Thulliez P, Remington JS. Differential agglutination test for diagnosis of recently acquired infection with Toxoplasma gondii. J Clin Microbiol 28:1928-1933, 1990

• Reference range: high avidity excludes infection within the previous 4 months
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: 86777-59
• Fee: $196 (Includes fee for Remington IgG (Dye test) and Remington IgM ELISA)
• Method Reference: Pelloux, H. et al. Determination of anti-Toxoplasma gondii immunoglobulin G avidity: adaption to the Vidas system (bioMérieux). Diagn Microbiol Infect Dis 32: 69-73, 1998

• Reference range: reported as "toxoplasma DNA detected" or "toxoplasma DNA not detected"
• Specimen: (refer to PCR Specimen Requirements table)
• Volume: (refer to PCR Specimen Requirements table)
• Transport temperature: (refer to PCR Specimen Requirements table)
• CPT code: 87798
• Fee: $465-$485 (refer to Fee Schedule)
• Method Reference: Grover C, Thulliez P, Remington J, Boothroyd J. Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. J Clin Microbiol 28:2297-2301, 1990.
  
  Parmley S, Goebel F, Remington JS. Detection of Toxoplasma gondii in cerebro-spinal fluid from AIDS patients by polymerase chain reaction. J Clin Microbiol 30:3000-3002, 1992

• Reference range: reported as positive or negative
• Specimen: whole blood (EDTA or Citrate preferred), amniotic fluid, placenta and other tissues
  
  NOTE: Specimens must not be frozen or placed in formalin or other fixative. Inoculations are recommended within 48 hours of specimen collection date. Results available in six weeks.
• Volume: 3 ml (fluids); 1 g (solid tissues)
• Transport temperature: 2 – 8° C.
• CPT code: 87003
• Fee: $677
• Method Reference: Remington J.S., McLeod R., Thulliez P., Desmonts G. Toxoplasmosis.
  

  
  In Remington J.S., Wilson C.B., Baker C.J. and Klein, J.O. (eds.) Infectious Diseases of the Fetus and Newborn Infant, Sixth Edition. Philadelphia: Elsevier Saunders Company, 2006, pp 1009-1010.

• Reference range: reported as negative or positive
• Specimen: serum
• Volume: 0.5 ml
• Transport temperature: 2 – 8° C (preferred); ambient (acceptable)
• CPT code: 86318
• Fee: $140
• Method Reference: Montoya JG, Adams AE, Bonetti V, Deng S, Link NA, Pertsch S, Olson K, Li M, Dillon EC, Frosch DL. 2021. Differences in IgG antibody responses following BNT162b2 and mRNA-1273 SARS-CoV-2 vaccines. Microbiol Spectr 9:e01162-21.
  
  Renard N, Daniel S, Cayet N, Pecquet M, Raymond F, Pons S, Lupo J, Tourneur C, Pretis C, Gerez G, Blasco P, Combe M, Canova I, Lesenechal M, Berthier F. 2021. Performance characteristics of the Vidas SARS-CoV-2 IgM and IgG serological assays. J Clin Microbiol 59:e02292-20.

• Reference range: reported as negative or positive
• Specimen: nasal swab, nasopharyngeal swab, oropharyngeal swab
• Volume: swab in transport media (viral transport media preferred)
• Transport temperature: ambient; 2 – 8° C
• CPT code: 87635
• Fee: $155

Method Reference: TaqPath™ COVID-19 Combo Kit INSTRUCTIONS FOR USE, Catalog Number A47814 Publication Number MAN0019181 Revision G.0

IgG (Dye Test), Remington IgM ELISA,  Alternative IgG, Alternative IgM

• Specimen: serum
• Volume: 0.5 to 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $350

IgG (Dye Test), Remington IgM ELISA, Avidity

• Specimen: serum
• Volume: 0.5 to 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $546

IgG (Dye Test), Remington IgM ELISA, AC/HS, Avidity

• Specimen: serum
• Volume: 0.5 to 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $742

IgG (Dye Test), Remington IgM ISAGA, Remington IgA ELISA

• Specimen: serum
• Volume: 1.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $515

IgG (Dye Test), Remington IgM ELISA, Remington IgA ELISA, Remington IgE ELISA, AC/HS

• Specimen: serum
• Volume: 2.0 ml
• Transport temperature: 2 – 8° C. (preferred); ambient or frozen (acceptable)
• CPT code: Refer to individual tests above
• Fee: $876